Cell and nuclear extraction are vital techniques to isolate cellular components for analysis. These methods are applicable to both adherent and suspension cells. Adherent cells are scraped off their growth surface, while suspension cells are collected by centrifugation. Both types of cells undergo lysis and centrifugation to yield cytoplasmic and nuclear fractions. These high-quality extracts are essential for various research applications, including protein quantification and signal transduction studies.
This protocol has been developed for rapid isolation of cytokines and signaling molecules, using a non-abrasive extraction reagent for total protein extraction. The method is sensitive and allows for the detection of disease-associated biomarker fluctuations. The prepared extracts are compatible with a variety of immunoassays, including ELISA and the Luminex platform, as well as conventional protein assays. Typically, this extraction method is cost-effective, versatile, and can be completed in less than 15 minutes.
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